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Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression

2013Journal article Open access
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dc.contributor.authorWatson, Emma L.
dc.contributor.authorKosmadakis, George C.
dc.contributor.authorSmith, Alice C.
dc.contributor.authorViana, Joao L.
dc.contributor.authorBrown, Jeremy R.
dc.contributor.authorMolyneux, Karen
dc.contributor.authorPawluczyk, Izabella Z. A.
dc.contributor.authorMulheran, Michael
dc.contributor.authorBishop, Nicolette C.
dc.contributor.authorShirreffs, Susan
dc.contributor.authorMaughan, Ronald J.
dc.contributor.authorOwen, Paul J.
dc.contributor.authorJohn, Stephen G.
dc.contributor.authorMcIntyre, Christopher W.
dc.contributor.authorFeehally, John
dc.contributor.authorBevington, Alan
dc.date.accessioned2021-04-28T14:41:06Z
dc.date.available2021-04-28T14:41:06Z
dc.date.issued2013
dc.description.abstractMuscle-wasting in chronic kidney disease (CKD) arises from several factors including sedentary behaviour and metabolic acidosis. Exercise is potentially beneficial but might worsen acidosis through exercise-induced lactic acidosis. We studied the chronic effects of exercise in CKD stage 4-5 patients (brisk walking, 30 min, 5 times/week), and non-exercising controls; each group receiving standard oral bicarbonate (STD), or additional bicarbonate (XS) (Total n = 26; Exercising + STD n = 9; Exercising +XS n = 6; Control + STD n = 8; Control + XS n = 3). Blood and vastus lateralis biopsies were drawn at baseline and 6 months. The rise in blood lactate in submaximal treadmill tests was suppressed in the Exercising + XS group. After 6 months, intramuscular free amino acids (including the branched chain amino acids) in the Exercising + STD group showed a striking chronic depletion. This did not occur in the Exercising + XS group. The effect in Exercising + XS patients was accompanied by reduced transcription of ubiquitin E3-ligase MuRF1 which activates proteolysis via the ubiquitin-proteasome pathway. Other anabolic indicators (Akt activation and suppression of the 14 kDa actin catabolic marker) were unaffected in Exercising + XS patients. Possibly because of this, overall suppression of myofibrillar proteolysis (3-methylhistidine output) was not observed. It is suggested that alkali effects in exercisers arose by countering exercise-induced acidosis. Whether further anabolic effects are attainable on combining alkali with enhanced exercise (e.g. resistance exercise) merits further investigation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00421-013-2628-5pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.24/1770
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAdultpt_PT
dc.subjectAgedpt_PT
dc.subjectAged, 80 and overpt_PT
dc.subjectAmino Acidspt_PT
dc.subjectBicarbonatespt_PT
dc.subjectCombined Modality Therapypt_PT
dc.subjectFemalept_PT
dc.subjectHumanspt_PT
dc.subjectLactic Acidpt_PT
dc.subjectMalept_PT
dc.subjectMiddle Agedpt_PT
dc.subjectMuscle Proteinspt_PT
dc.subjectMuscle, Skeletalpt_PT
dc.subjectProto-Oncogene Proteins c-aktpt_PT
dc.subjectRenal Insufficiency, Chronicpt_PT
dc.subjectTripartite Motif Proteinspt_PT
dc.subjectUbiquitin-Protein Ligasespt_PT
dc.subjectExercise Therapypt_PT
dc.subjectWalkingpt_PT
dc.titleCombined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expressionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2124pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage2111pt_PT
oaire.citation.titleEuropean Journal of Applied Physiologypt_PT
oaire.citation.volume113pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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